To accurately control the freezing rate, a homemade equipment was designed according to a previous report (11). The equipment includes an automatic liquid nitrogen tank, frozen ethanol pool, sample stage (copper cylinder, diameter of 10 cm), and a temperature controller with a heating jacket wrapped the copper cylinder (fig. S4). Ethanol was used as a buffer of the liquid nitrogen due to its low melting point. The temperature controller can show the real-time temperature of the sample stage, maintain steady temperature, and control the freezing rate. Before the sample preparation, the temperature of the sample stage was maintained at −10°C. After adding the solution to the mold, the temperature of the sample stage was controlled to decrease at a constant rate. After the samples were completely frozen, the mold was taken down and the samples were taken out carefully. Note that any possible impact should be avoided, or else cracks will appear on the final samples.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.