We analyzed audio files using Avisoft-SASLab Pro software. We inspected all four audio channels, beginning 900 ms before the selected interaction, and analyzed the channel with the highest signal-to-noise ratio [following (54)]. Since free-flight interactions occurred at unpredictable locations around the room, recordings of bats echolocating far or off-axis from the microphones resulted in low signal-to-noise ratios and were thus discarded from the analysis. When all echolocation calls of an interaction were distinct from background noise, we manually extracted buzz I and buzz II durations from the oscillogram. We did not assess frequency components of the calls, as the variable positioning of the bats in relation to the microphones would likely lead to inaccurate frequency data due to the effects of off-axis directionality and atmospheric attenuation.

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