lin-65 (n3441) moms were crossed with JAC500 his-72::mCherry males. Progeny of lin-65 +/− heterozygotes marked by mCherry were analyzed. On the same slide, SM2333 containing a single copy of zen-4::gfp was used as a WT staining control. Mean H3K9me2 or H3K9me3 intensity in each nucleus was quantified using Volocity, and the average H3K9me2 or H3K9me3 intensity per nucleus was plotted and normalized as described in the “Quantitation of histone modifications by Volocity” section.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.