lin-65 (n3441) moms were crossed with JAC500 his-72::mCherry males. Progeny of lin-65 +/− heterozygotes marked by mCherry were analyzed. On the same slide, SM2333 containing a single copy of zen-4::gfp was used as a WT staining control. Mean H3K9me2 or H3K9me3 intensity in each nucleus was quantified using Volocity, and the average H3K9me2 or H3K9me3 intensity per nucleus was plotted and normalized as described in the “Quantitation of histone modifications by Volocity” section.

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