Worms were maintained at 20°C according to Brenner (30), unless stated otherwise:

(1) N2 (WT, Bristol).

(2) RB1789 met-2(ok2307) III, provided by the C. elegans Gene Knockout Project at Oklahoma Medical Research Foundation.

(3) MT13232 lin-65(n3441) I (20).

(4) SM2078 stIs10389 (pha-4::gfp::3xflag); pha-4 (q500) rol-9 (sc148) (31), integrated array strain.

(5) SM2333 pxSi01 (zen-4::gfp, unc-119+) II; unc-119(ed3) III, generated by Mos1-Mediated Single-Copy Insertion (MosSCI).

(6) SM2529 arle-14/B0336.5(tm6845) III, provided by the Japanese National Bioresource Project.

(7) JAC500 his-72(csb43[his-72::mCherry]) III, provided by Norris et al. (32).

(8) EL597 omIs 1 [Cb-unc-119 (+) met-2::gfp II], generated by MosSCI.

(9) SM2491 omIs 1 [Cb-unc-119 (+) met-2::gfp II]; met-2(ok2307) unc-119 (ed3) III.

(10) SM2533 omIs1 [Cb-unc-119 (+) met-2::gfp II]; arle-14(tm6845) III.

(11) SM2536 omIs1 [Cb-unc-119 (+) met-2::gfp II]; lin-65 (n3441) I.

(12) SM2532 [Cb-unc-119 (+) met-2::gfp II]; his-72(csb43[his-72::mCherry]) III.

(13) EL634 3xflag::met-2 III. This study, generated by CRISPR.

(14) SM2575 lin-65::3xflag I. This study, generated by CRISPR.

(15) SM2576 arle-14(tm6845) III, lin-65::3xflag I. This study.

(16) SM2578 met-2(ok2307) III, lin-65::3xflag I. This study.

(17) SM2580 NLS::3xflag::met-2 III. This study, generated by CRISPR.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.