Transgenic and WT plantlets were propagated in vitro and transferred to soil as described previously (38). Cassava was cultivated under glasshouse conditions (60% humidity, 26°C, and 16-hour supplemental lighting) and fertilized fortnightly (Wuxal Universal Fertilizer). Plants were harvested after 6 months growth. Harvesting was performed toward the end of the photoperiod to maximize starch yields. Plant height (base of stem to apical shoot tip) was measured, and leaf numbers 1, 2, and 4 (counted from apical leaf) were isolated and frozen in liquid nitrogen before they were ground to a fine powder using pestle and mortar. The root mass of each plant was removed and weighed (in grams). The two largest storage roots from each plant were isolated, peeled, chopped, and briefly rinsed in water before they were frozen in liquid nitrogen and ground to a fine powder (IKA Analytical Mill). Tissue samples were stored in sterile vessels at −80°C.

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