Reverse transcription for individual qPCRs was carried out using 250 ng of total RNA and the High-Capacity Reverse Transcription Kit (no. 4368814, Life Technologies) according to the manufacturer’s instruction. qPCRs were run on the ABI 7900HT Fast Real-Time PCR instrument (Applied Biosystems). Gene expression analysis was conducted using a relative standard curve method with glyceraldehyde-3-phosphate dehydrogenase to normalize the expression levels of target genes. All reactions were performed in triplicates.

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