The isolation of total RNA was performed using the miRNeasy Mini kit (QIAGEN, no. 217004) according to the manufacturer’s instructions. The sample quality was determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and Agilent 2100 Bioanalyzer. GeneChip Mouse Gene 2.1 ST arrays (Affymetrix, Santa Clara, CA; no. 902120) were used for mRNA profiling. Total mRNA (100 ng) was used for complementary DNA synthesis using the Ambion WT Expression Kit (Life Technologies, no. 4411974). Hybridization, washing, and scanning were conducted according to the Affymetrix guidelines for the GeneAtlas instrument.

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