The isolation of total RNA was performed using the miRNeasy Mini kit (QIAGEN, no. 217004) according to the manufacturer’s instructions. The sample quality was determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and Agilent 2100 Bioanalyzer. GeneChip Mouse Gene 2.1 ST arrays (Affymetrix, Santa Clara, CA; no. 902120) were used for mRNA profiling. Total mRNA (100 ng) was used for complementary DNA synthesis using the Ambion WT Expression Kit (Life Technologies, no. 4411974). Hybridization, washing, and scanning were conducted according to the Affymetrix guidelines for the GeneAtlas instrument.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.