Ten mice were implanted with a telemetry probe [ETA-F10, Data Science International (DSI), St Paul, MN] for electroencephalography (EEG) analysis only (the brain was not harvested for transcriptome or connectome analysis). The stereotaxic surgery was performed under ketamine (100 mg/kg)/xylazine (10 mg/kg) anesthesia. Lidocaine was used locally. Temperature, heart rate, and breathing rate were continuously monitored during the surgical procedure using MouseOx Plus monitor (Starr Life Sciences Corp., Oakmont, PA, USA). A recording skull screw was secured above the hippocampal CA1 region (−1.8 mm posterior, +1.8 mm lateral), and a reference skull screw was secured above the cerebellum. The leads of the DSI telemetry probe (DSI, St Paul, MN) were wrapped around the recording and reference screws, and the screws were all encased in dental cement. The probe body containing the battery was subcutaneously placed in the back of the mice. Animals were allowed 7 days of postsurgical recovery before any further experimental procedures were conducted. EEG recordings were continuously performed until the end of the experimental protocol. Seizures were automatically detected with a DSI software (adjusting for frequency and amplitude) using low detection values. This led to numerous false positives (mostly movement artifacts), which were manually removed after double checking by a trained technician and clinical epileptologist. Complete EEG recordings were fully visually inspected. No false negatives were detected (i.e., the semi-automated procedure insured the detection of all seizures). All pilocarpine-treated mice developed spontaneous seizures.

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