Adult FVB mice were injected with methylscopolamine [1 mg/kg, intraperitoneally (ip)] 30 min before the pilocarpine injections. Pilocarpine was repeatedly injected (100 mg/kg, ip), every 20 min, until status epilepticus (SE) was observed. After 90 min of SE, we injected diazepam (10 mg/kg, ip) to stop SE. All mice then received 0.5 ml of NaCl (0.9%) subcutaneously and again in the evening. During the following days, if required, then mice were fed using a syringe.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.