Adult FVB mice were injected with methylscopolamine [1 mg/kg, intraperitoneally (ip)] 30 min before the pilocarpine injections. Pilocarpine was repeatedly injected (100 mg/kg, ip), every 20 min, until status epilepticus (SE) was observed. After 90 min of SE, we injected diazepam (10 mg/kg, ip) to stop SE. All mice then received 0.5 ml of NaCl (0.9%) subcutaneously and again in the evening. During the following days, if required, then mice were fed using a syringe.

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