All experiments were performed on FVB adult male mice following INSERM procedures. All animals had the same age (less than 1 week difference), and tissue collection was performed when animals were 14 weeks old. Twenty-four control and 24 TLE mice were used for transcriptomics and proteomics. They were kept in a special in-house animal facility with a strict control of light and temperature conditions (beginning of the light phase at 7:30 and beginning of the night phase at 1930). A red light, which does not disrupt circadian rhythmicity, was present during the night phase to allow researchers to manipulate the animals. During the night phase, no external light could enter the room when opening the door. Mice were housed in groups of four to five to enable social interaction. Cages had enriched environment. Throughout the experimental procedure, the same researcher took care of the animals at the same time of the day to limit external stressful factors. Animals were anesthetized with isoflurane in the animal facility, and four of them were sacrificed every 4 hours (six time points).

The brain was quickly extracted, and the hippocampus was removed in modified ice-cold artificial cerebrospinal fluid (ACSF). Right (for transcriptome analysis) and left (for proteomics) extracted hippocampi were separated into two halves, i.e., ventral and dorsal parts, and quickly frozen. Only the ventral parts were used here. The average time between decapitation and sample freezing was 90 s per mice to limit any degradation of gene products and proteins. All tissue collection was performed during a single 24-hour period. The collection of the hippocampal tissue from the four mice per time window was performed in less than 10 min.

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