Cells were fixed with 2% glutaraldehyde in 100 mM phosphate buffer (pH 7.2) for 2 hours at 26°C, postfixed with 2% potassium permanganate overnight at 4°C, and embedded in Epon812 (TAAB). Ultrathin sections were stained in 2% uranyl acetate and Reynold’s lead citrate and viewed with a TEM JEM1230R (JEOL) operated at 100 kV.

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