Strain RRG112 was generated by transforming plasmids pRRG13 (Kmr) and pRRG62 (Ampr) into E. coli BW27783 by electroporation. Strains RRS113 and RRS129 were generated by transforming plasmids pRRG63 (Kmr, Ampr) and pRRG74 (Ampr), respectively, into E. coli BW27783 by electroporation. Strain RRS247 was generated by inserting the region comprising the araC to km genes (both included) from plasmid pRRG63 into E. coli chromosome. This way, strain RRS247 is the chromosomal counterpart of strain RRS113 (fig. S1A). The DNA fragment was PCR amplified from RRS113 using primers AraC_Wanner and Km_AraD_Wanner. These primers contained a homologous region to the ara operon such that the amplified fragment could be recombined into E. coli TB10 strain following the protocol described in (38). A P1 lysate was then prepared and transduced to strain BW27783 to yield strain RRS247.

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