FRET signal in live cells was detected by an EnVision 2104 Multilabel Plate Reader (PerkinElmer Life Sciences). The reaction was excited by light passing through a 430-nm filter (with 8 nm bandwidth), and the intensity of emitted fluorescence passing through a 530-nm filter (with 8 nm bandwidth) was recorded. If the protein-protein pair was in close proximity (1 to 10 nm), a 530-nm signal would be detected. The FRET efficiency was calculated by a formula, efficiency = (1 − FDA/FD) × 100% (where FDA is the relative fluorescence intensities of the donor in the presence of the acceptor and FD is the relative fluorescence intensities of the donor in the absence of the acceptor).

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