The 2-kb IL-17A promoter–driven firefly luciferase reporter plasmid and a Renilla luciferase control plasmid (pRL-TK) were cotransfected into Jurkat T cells. After 24 hours, 1 × 106 cells were harvested, washed with phosphate-buffered saline, and resuspended in 60 μl of RPMI 1640 plus 60 μl of lysis buffer. Data represent the mean of the ratios of the firefly luciferase activity to the Renilla luciferase activity.

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