KG1a cells, a human acute myelogenous leukemia cell line, purchased from the American Type Culture Collection, were maintained in Iscove’s modified Dulbecco’s medium (IMDM; Gibco) supplemented with 20% fetal bovine serum, penicillin (100 U ml−1), and streptomycin (100 μg ml−1) at 37°C in a humidified atmosphere containing 5% CO2. For the disruption of actin cytoskeletons, 106 ml−1 cells were treated with cytochalasin D (CytD; 3 μg ml−1; Sigma) in a prewarmed Hanks’ balanced salt solution (HBSS; Gibco) for 30 min at 37°C. For the disruption of lipid rafts, 106 ml−1 cells were treated by a prewarmed 10 mM MβCD (Sigma) and 10 mM Hepes buffer (Sigma) in a serum/antibiotic-free IMDM at 37°C for 30 min. The viability of the cells after the treatments was confirmed by trypan blue staining.

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