Household dish soap was diluted 1:10 in water along with 1 g of table salt and then added to a plastic bag containing chopped fruit (banana, kiwi, or strawberry). The fruit was then gently crushed in the soap and salt mixture by hand until a homogeneous mixture was obtained. The resulting mixture was strained through a household coffee filter into a cup. A prechilled 25-ml volume of 91% isopropyl alcohol (rubbing alcohol) was added to the strained liquid. The mixture was left undisturbed for 5 min to allow phase separation to occur. The upper white layer containing extracted DNA was removed, placed on a clean coffee filter, and washed with 70% ethanol (ethyl rubbing alcohol). The resulting extracted DNA was then patted with paper towels to remove any excess extraction liquid. The DNA was then diluted in water until it dissolved and added to an isothermal RPA, according to the manufacturer’s protocol (TwistAmp Basic RT, TwistDx; fig. S5), with primers that were complementary to one section of the banana or kiwi genome (table S1). The primers also incorporated a T7 promoter for transcription in FD-CF. The resulting RPA product was then added 1:3.75 to a rehydrated FD-CF reaction containing a linearized toehold complementary to the amplified RPA product and run according to the FD-CF methods described above.

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