Light microscopy imaging was used to define areas of interest for Raman microscopy. Optical imaging was performed with an Olympus BX61 microscope coupled to computer control using Olympus cellSens software. Maps of the samples were prepared using both transmission and reflected light at ×40 and ×100 using the multiple image alignment function of the microscope. These maps were then used to locate areas for CRIS imaging and FIB milling.

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