Specimen DIP-S-0907 was scanned with a MicroXCT 400 (Carl Zeiss X-ray Microscopy Inc.) at the Institute of Zoology, Chinese Academy of Sciences. The entire animal (Fig. 1) was divided into seven scans that were combined to create a single model, and the scans were conducted with a beam strength of 60 kV, 8 W, and absorption contrast and a spatial resolution of 2.5464 μm. In addition, specimen DIP-S-0907 was imaged using propagation phase-contrast synchrotron radiation microtomography on the beamline 13W at the Shanghai Synchrotron Radiation Facility. The isotropic voxel size was 2.25 μm.

On the basis of the obtained image stacks, structures of the specimen were reconstructed and separated with Amira 5.4 (Visage Imaging). The subsequent volume rendering was performed with Avizo 9.0 (Thermo Fisher Scientific) and VG Studiomax 2.1 (Volume Graphics). The neonate C. ruffus was loaned from the Western Australian Museum (WAM R49553) and scanned with a SkyScan 1076 (Bruker MicroCT) at Adelaide Microscopy, University of Adelaide, Australia. The scan settings were 65 kV, 153 μA, no filter, and an isotropic voxel size of 8.7 μm. The reconstruction was carried out using the software NRecon (Bruker MicroCT), and the volume renderings were created in the software Avizo 9.0 (Thermo Fisher Scientific).

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