Tissue samples were resected, snap-frozen, and kept at −80°C. Blood (500 μl) was collected in Capiject tubes (Terumo), left at room temperature for 20 min, and centrifuged at 1200g for 10 min. The serum was collected, snap-frozen, and kept at −80°C. For each tissue sample, 100 mg of tissue was homogenized in 1 ml of PBS and diluted by 1:250 (lung and intestine) or 1:5000 (liver) with PBS before measurements. Mouse VEGF-C levels were measured using a mouse VEGF-C ELISA kit (Cusabio), following the manufacturer’s instructions.

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