Nicotine hydrogen tartrate salt was dissolved in 0.9% sterile physiological sodium chloride and prepared fresh every other day (pH 7.3). The dose of nicotine for self-administration was 0.03 mg/kg per 100 μl. Yohimbine HCl was dissolved in distilled water and injected intraperitoneally at a dose of 2 mg/kg. NicA2-J1 was synthesized as previously reported (19) and administered intraperitoneally at doses of 2 and 10 mg/kg.

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