The microhelices were mixed with 20-μm silica microparticles (~0.02%; sicastar-greenF, micromod Partikeltechnologie GmbH). A ~3-mm incision was made, and 100 μl of solution was injected into the center of the vitreous using a 100-μl pipette. The eye was then placed at the center of a custom-built three-axis Helmholtz coil setup. A rotational magnetic field with a strength of 8 mT and a frequency of 70 Hz was applied. The rotational axis was pointed toward the optic disc region of the retina, and the rotational direction was counterclockwise (viewing from the side of the lens). The eye was then placed on the imaging instrument that combines scanning laser imaging and high-resolution OCT [SPECTRALIS Heidelberg Engineering Retinal Angiography (HRA)+OCT, Heidelberg Engineering GmbH] by a custom holder. Fluorescent silica particles were imaged under a 485-nm laser excitation and a 510-nm emission filter in the HRA mode. The slippery micropropellers were imaged by OCT scans with both 19 slices in X and Y directions, respectively. The experiment was independently repeated three times. Counting of micropropellers in each OCT scan was conducted, and pseudocolor images were generated by a custom script in MATLAB (R2017a, MathWorks).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.