MDCKII and TIF cells were fixed in 4% paraformaldehyde for 20 min at room temperature. The scaffolds were washed extensively with PBS and incubated with rhodamine phalloidin (Sigma) for 30 min to label actin filament. Fluorescence images of the scaffolds were obtained using an epifluorescence/confocal microscope (Axio Observer Z1 LSM 800, Zeiss).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.