MDCKII and TIF cells were fixed in 4% paraformaldehyde for 20 min at room temperature. The scaffolds were washed extensively with PBS and incubated with rhodamine phalloidin (Sigma) for 30 min to label actin filament. Fluorescence images of the scaffolds were obtained using an epifluorescence/confocal microscope (Axio Observer Z1 LSM 800, Zeiss).

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