SET-2 cells were plated in MethoCult H4435 (STEMCELL Technologies) after 48 hours of drug treatment, followed by washout in low FCS media and low-dose cytokines (24 hours) and scored for colony-forming unit blast at 10 days. Primary CD34+ stem/progenitor cells from peripheral blood samples from patients with JAK2V617F mutant positive myelofibrosis were purified by flow cytometry using anti–CD34-APC (clone 8G12), anti-human CD45RA Brilliant Violet 605, anti-CD90 fluorescein isothiocyanate, anti-human CD123-phycoerythrin (PE), and anti-human CD38 PE-Cy7 and plated in MethoCult H4435 at 1000 CD34+ per plate after ruxolitinib treatment and washout.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.