To enhance parasitemia and simultaneously induce gametocytemia early after infection as previously published (8), erythropoiesis was stimulated by the intraperitoneal injection of 200 μl of a solution of phenylhydrazine in saline (6 mg/ml). Mice were infected (intraperitoneally) with 105 Pb ANKA-mCherryHsp70, G623, G629, G488, or G458 parasites 2 days later. Blood smears were made daily and checked both by fluorescence and Wright staining. Upon reaching 5 to 10% parasitemia, to suppress proliferation of asexual stage parasites, mice received sulfadiazine (10 mg/liter) in their drinking water (from days 3 to 4) after infection onward. Sulfadiazine treatment led to elimination of asexual populations. Because gametocytemia decreases also after 4 or more days of sulfadiazine treatment, IVM, bioluminescence, or collection of infected blood for the purification and passage of gametocytes was routinely performed after 3 to 4 days of treatment (that is, 6 to 7 days after infection).

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