TF1.8 or SET-2 cells were starved for 3 hours in RPMI containing 0.5% (v/v) FCS and then plated at 1.5 × 105 cells/ml with the addition of human IL-3 (2 ng/ml) in the presence of either 0.1% (v/v) DMSO or a titration of ruxolitinib or CHZ868. Cells were analyzed for apoptosis after 48 hours by staining with 1:100 annexin V–allophycocyanin (APC) in 1× annexin V binding buffer (BD Pharmingen) for 20 min at 4°C. The percentage of cells that were annexin V positive was determined by flow cytometry using an LSRFortessa Special Order Research Flow Cytometer with FACSDiva Software version 8.0 (BD Biosciences, San Diego, CA, USA). Data are expressed as means ± SEM from triplicates, and n = 3 independent experiments.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.