TF1.8 or SET-2 cells were resuspended in RPMI supplemented with 10% (v/v) FCS, plated at 2 × 104 cells per well in 96-well plates, and incubated with titrations of cytokine or inhibitors for 2 days. Cell proliferation was assessed using CellTiter 96 AQueous (#G3581, Promega) following the manufacturer’s protocol. Data are expressed as means ± SEM from triplicates, and n = 3 independent experiments.

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