TF1.8 cells were cultured in RPMI with 10% (v/v) FCS supplemented with 10 mM Hepes and GM-CSF (2 ng/ml). SET-2 cells were cultured in RPMI with 10% (v/v) FCS. γ2A cells were cultured in Dulbecco’s modified Eagle’s medium with 10% (v/v) FCS.

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