Mice were anesthetized with isoflurane and euthanized by cervical dislocation. Pancreatic islets were prepared by retrograde injection of a collagenase solution via the biliopancreatic duct and digestion at 37°C for 18 min. Liberated islets were handpicked under a stereomicroscope at room temperature. Before use, the islets were cultured in RPMI1640 medium containing 11 mM glucose, 10% (v/v) FBS, penicillin (100 IU/ml), and streptomycin (100 μg/ml; Sigma, #P4333). Islets were dispersed using TrypLE Express (Thermo Fisher Scientific, #12605036) and plated in 96-well PDL-coated plates.

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