β-Arrestin recruitment was measured in Chinese hamster ovary (CHO) K1 hGLP1R β-arrestin2 (DiscoverX, #93-0300C2) cells after 90 min incubation with compounds in Ham’s F-12, 1% FBS, and 0.3% DMSO at 37°C according to the manufacturer’s PathHunter assay protocol (DiscoverX Corporation, Fremont, CA).

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