cAMP accumulation was measured in GLP1R-HEK293 cells using the cAMP Dynamic 2 Kit from Cisbio Bioassays (Colodet, France). Cells were incubated with compounds for 30 min at room temperature in HBSS supplemented with 20 mM Hepes (pH 7.4), 0.1% BSA, and 1 mM 3-isobutyl-1-methylxanthine, followed by detection with homogeneous time-resolved fluorescence according to the manufacturer’s assay protocol.

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