DMR was measured using an Epic biosensor system (Corning). GLP1R-HEK293 and wild-type HEK293 cells were cultured in DMEM with 10% FBS in fibronectin-coated 384-well Epic plates (Corning, #5042) at 37°C, 95% humidity, and 5% CO2 for 24 hours. Following the incubation, culture medium was replaced with HBSS (Invitrogen), 20 mM Hepes (Invitrogen) (pH 7.4), 0.1% BSA (Sigma, #A6003), and 0.04% dimethyl sulfoxide (DMSO) and equilibrated to 26°C in the Epic system for 30 min before addition of compounds and detection of DMR.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.