DMR was measured using an Epic biosensor system (Corning). GLP1R-HEK293 and wild-type HEK293 cells were cultured in DMEM with 10% FBS in fibronectin-coated 384-well Epic plates (Corning, #5042) at 37°C, 95% humidity, and 5% CO2 for 24 hours. Following the incubation, culture medium was replaced with HBSS (Invitrogen), 20 mM Hepes (Invitrogen) (pH 7.4), 0.1% BSA (Sigma, #A6003), and 0.04% dimethyl sulfoxide (DMSO) and equilibrated to 26°C in the Epic system for 30 min before addition of compounds and detection of DMR.

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