All data were analyzed using GraphPad Prism 5. Numerical data were expressed as the mean ± SEM. Statistical significance was determined using Student’s t test for comparisons between two groups or analyses of variance (ANOVAs) for comparisons among three or more groups. One-way ANOVA and two-way ANOVA were followed by Bonferroni post hoc test. In all cases, n refers to the number of animals. For all results, P < 0.05 was considered statistically significant.

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