After injection with the AAV8-CaMKIIα-eNpHR-eYFP virus in the BLA or the PrL for a minimum of 6 weeks, mice were habituated to connect the mimical optical receivers (Teleopt, Japan) in their home cages (3 days, 5 min/day). As shown in the left panel of Fig. 2B, the pre-test (day 1), drug treatment (days 2 to 6), and conditioning (days 7 to 10) phases were similar as described above. On post-test day (day 11), the wireless optical receivers were connected to chronically implanted optical fiber and suspended above the behavioral testing arena to allow mice to move freely while receiving laser stimulation. The wireless optical receivers were controlled by a remote controller. All laser output was manipulated with a stimulator (Teleopt, Japan) to output TTL Hi signal. The mice with the expression of eNpHR-eYFP or eYFP were divided into two groups: light ON and light OFF. For mice in the light ON group, optogenetic inhibition was achieved through the optical fiber connected to the wireless optical receivers by using 10 mW (~35.35 mW/mm2) of 15 min of constant 594-nm light. For mice in the light OFF group, no light was given.

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