This protocol is extracted from research article:
Autonomous microfluidic actuators for periodic sequential flow generation
Sci Adv, Apr 19, 2019; DOI: 10.1126/sciadv.aat3080

For flow visualization, we used a camera (model u-Nova20C, Novitec) on a microscope (model Ti-U, Nikon) to capture the red, green, and blue (RGB) images under different filters. In Fig. 4C (Fig. 4D), BF and RF (GF and RF) intensities were measured using DAPI (4′,6-diamidino-2-phenylindole) and TRITC (4′,6-diamidino-2-phenylindole and tetramethyl rhodamine isothiocyanate) [FITC (fluorescein isothiocyanate) and TRITC] filters, respectively, and collated. To characterize the fluorescent intensity of cell nuclei, a scientific Complementary Metal-Oxide-Semiconductor (sCMOS) camera (pco.edge 5.5, PCO) was used to capture the gray-scale image of the cell nuclei. Pressure sensors (PX309-015GI, Omega) and a pressure generator (AF1 Dual, Elveflow) were used to measure the pressure of the devices and the duration times of solution movement. The emission wavelength was measured by a fluorescence spectrophotometer (Cary Eclipse, Agilent).

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