In vivo antitumor therapy with CLP nanoparticles after intratumoral delivery
This protocol is extracted from research article:
A self-illuminating nanoparticle for inflammation imaging and cancer therapy
Sci Adv, Jan 9, 2019; DOI: 10.1126/sciadv.aat2953

A549 xenografts in BALB/c nude mice were established as aforementioned. When the tumor volume reached ~100 mm3, mice were randomly divided into two groups (n = 6). The saline group was treated with 0.1 ml of saline, while animals in the CLP group were treated with 0.1 ml of saline containing CLP nanoparticles at 3.25 mg/kg of Ce6 by intratumoral injection every 3 days. The first day of intratumoral injection was considered as day 1. After administration, body weight and tumor size were monitored every 3 days for a total of 30 days. Tumor volume was estimated by the formula a × b2/2, where a and b refer to the major and minor axes of the tumor measured by a caliper, respectively. After treatment, mice were euthanized to collect blood for serum biochemistry assays. Tumor tissues and major organs were also harvested. Histological sections were prepared for H&E staining and detection of apoptosis by TUNEL assay. Immunofluorescence analysis was also performed by staining sections with FITC-labeled PCNA antibody and DAPI.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.