Acute liver injury in mice was established by intraperitoneal injection of acetaminophen at 300 mg/kg. After 24 hours, mice were randomly divided into three groups. Mice in the saline group were intravenously administered with 0.1 ml of saline. The CLP group was treated with intravenous injection of 0.1 ml of saline containing CLP nanoparticles (50 mg/ml) in each mouse, while the luminol group was administered with the same dose of the luminol unit. Immediately after injection, in vivo bioluminescence imaging was performed. In addition, ex vivo images of isolated liver tissues were acquired.

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