A549 cells were seeded in a 12-well plate at 1 × 105 cells per well for 12 hours. After incubation with CLP nanoparticles at various doses for 24 hours, cells were collected, washed with PBS, and stained with annexin V–fluorescein isothiocyanate (FITC) and propidium iodide. Cells were further incubated for 15 min at room temperature in the dark. The percentage of apoptotic cells was quantified by flow cytometry.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.