Mouse melanoma cell B16F10, human breast cancer cell MCF-7, A549, mouse vascular aortic smooth muscle cell MOVAS, and RAW264.7 murine macrophage cells were seeded in a 96-well plate at 1 × 104 cells per well and incubated for 12 hours, respectively. Then, cells were treated with the medium containing CLP nanoparticles at various doses for 24 hours. Cell viability was quantified by the thiazolyl blue tetrazolium bromide (MTT) assay using a microplate reader (EMax Plus Microplate Reader, Molecular Devices, USA). The relative viability of the untreated controls was normalized to be 100%, while the medium served as the background control. According to the improved Karber’s method, IC50 values were calculated.

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