The generation of singlet oxygen (1O2) was characterized by chemical trapping 1O2 with DPA (67). The decrease in UV absorbance between 300 and 450 nm of the corresponding thermostable endoperoxide DPAO2 was detected. The decreased absorbance at 355 nm was quantified to analyze the amount of produced 1O2. Specifically, DPA was dissolved in a solvent mixture of acetonitrile and deuterium oxide [1:1 (v/v)] to obtain a saturated solution. The dose-dependent changes in the presence of 100 mM H2O2 before and after 6 hours of incubation were recorded. Subsequently, 20 μM CLP was incubated with various concentrations of H2O2 and a DPA stock solution. After 6 hours, absorption spectra were recorded on a UV-visible spectrometer.

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