Bat wingbeat selection criteria
This protocol is extracted from research article:
Biomechanics of hover performance in Neotropical hummingbirds versus bats
Sci Adv, Sep 26, 2018; DOI: 10.1126/sciadv.aat2980

Bat wingbeats were segmented for each individual by cross-correlating a template force trace. We first noted all wingbeats for which the bat was hovering and away from the flight chamber surfaces. The aerodynamic forces of all these wingbeats were included in the analysis, allowing us to determine an average representative force trace. Wingbeats were manually ranked on the basis of visibility in all three camera views. The wingbeat that most closely resembled hovering (low body roll and forward speed), and was visible in all views, was selected for kinematic tracking to minimize variability in hovering kinematics while not feeding. We excluded bats from the analysis for which we could not identify reasonably trackable wingbeats (three individuals). As not all noted bat wingbeats included a visible wing tip in every camera view, exact wingbeat transitions were calculated by extracting the vertical force trace of the single template wingbeat that was tracked kinematically. The maximum vertical position of the bat wing tip (37) was used to define the start of this wingbeat. The corresponding vertical force trace template for that kinematically tracked wingbeat was then cross-correlated with the full flight force trace. The maximum cross-correlation values defined the start and end of each bat wingbeat for aerodynamic force segmentation.

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