Data from included studies will be abstracted independently by two reviewers using a standardized and pretested form. In case of discrepancy, consensus will be reached through discussion or the involvement of a third reviewer. We will collect data on study characteristics (design, length of follow-up), sample characteristics (species, number, age, weight, gender, hemoglobin level), model preparation (anesthesia, type and severity of injury, hemorrhage, hemodilution), intervention (target, threshold, units, volume, timing with injury), co-interventions (crystalloids, colloids, medications, surgical interventions, etc.) and outcomes. We will use graph analysis tools to extract data of interest if results are not reported in the text or tables. If necessary, we will contact authors for additional information.

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