The heavy chain variable domains of selected antibodies were cloned into a modified pVRC8400 expression vector to produce a full-length human IgG1 heavy chain (39). IgGs were produced by transient transfection of 293F cells as specified above. Five days posttransfection, supernatants were harvested, clarified by low-speed centrifugation, and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column, washed with a column volume buffer A, and eluted in 0.1 M glycine (pH 2.5), which was immediately neutralized by 1 M Tris(hydroxymethyl)aminomethane (pH 8). Antibodies were then dialyzed against PBS, pH 7.4.

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