Human 293F cells were maintained at 37 °C with 5% CO2 in FreeStyle 293 Expression Medium (Thermo Fisher) supplemented with penicillin and streptomycin. High Five Cells (BTI-TN-5B1-4) (Trichoplusia ni) were maintained at 28 °C in EX-CELL 405 medium (Sigma) supplemented with penicillin and streptomycin.

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