The following antibodies were used for the indicated dilutions. For immunoblotting: rabbit anti-NGLY1 (1:100; ATLAS ANTIBODIES; HPA036825), mouse anti-GAPDH (6C5; 1:5000; Millipore; MAB374), and HRP-conjugated antibody (1:10,000; CST; 7076S). For immunostaining: rabbit anti-GFAP (1:2000; Abcam; ab7260), goat anti-IbaI (1:1000; Abcam; ab5076), rabbit anti-GSTP1 (1:300; GenTex; GTX112695), rabbit anti-NeuN (1:3000; Abcam; ab177487), and mouse anti-NeuN (1:1000; BioLegend; SIG-39860).

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