To assess changes in gait, gait analysis was carried out. The rats’ forepaws and hind paws were coated with two non-toxic water-soluble inks (forepaws in green, hind paws in red). The rats were then allowed to walk along a 100 cm long, 15 cm wide runway. The floor of the runway was covered with sheets of white paper. The footprints were manually analyzed, and the stride length of each paw and the stance ratio (width ratio; hind to forelimbs) were quantified.

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