The level of end product of lipid peroxidation (malondialdehyde [MDA] was measured through thiobarbituric acid reactive substances (TBARS) analysis [39]. Briefly, 20 µl of mitochondrial supernatants were added by 390 µL trichloroacetate (28%) and were centrifuged (3500 g for 15 min). Afterward, the supernatant was mixed with TBA (0.2% in sodium sulfate) on boiling water and incubated for 40 min, followed by the addition of n-butanol. Finally, the absorbance of samples was read at 532 nm using a microplate reader (BIO-RAD microplate reader-550).

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