Motor performance and coordination were tested using an accelerating rota-rod (Muromachi Kikai Co., Ltd.; MK610) at different ages. Each rat underwent 2 days of training sessions and a 1-day test session. On the training days, the rats completed four separate trials of 1 min each, walking at 4 rpm with a 20 min interval between trials. On day 3, the test was performed comprising four separate trials separated by 20 min intervals. During the test session, the speed of the rota-rod was accelerated from 4 to 40 rpm over the course of 4 min, and the time taken for the rats to fall from the rod was measured. If the rats stayed on the rod until the end of the 4 min trial, a time of 240 s was recorded.

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