The generation of intracellular ROS was investigated through the dichlorodihydrofluorescein diacetate (DCFH-DA) assay. Briefly, 20 µl of mitochondrial supernatants were incubated with a DCFH-DA probe (10 μM) for 30 min. Then, the fluorescence intensity of the samples was read at λexem of 485 nm/530 nm using a spectrofluorometer (CLARIOstar, Offenburg, Germany).

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