MTT assay was done to assess the mitochondria viability. 100 µl of mitochondrial supernatants were mixed by MTT stock solution (5 mg mL−1) for 4 h, followed by removing the supernatants, addition of 150 μL DMSO for 5 min, and reading the absorbance of the samples at 570 nm using a microplate reader (BIO-RAD microplate reader-550).

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