Immunohistochemistry
This protocol is extracted from research article:
Cytochrome P450 Omega-Hydroxylase 4a14 Attenuates Cholestatic Liver Fibrosis
Front Physiol, May 31, 2021; DOI: 10.3389/fphys.2021.688259

Mouse livers were fixed, dehydrated, and embedded in paraffin wax. Fixed liver sections (5 μm) were incubated with an anti-α-smooth muscle actin (anti-α-SMA) antibody (1:200), an anti-Col1a2 antibody (1:500), or an anti-smad3 antibody (1:100) overnight at 4°C and then with a polyperoxidase-conjugated goat anti-rabbit IgG (Zhongshan Golden Bridge, Beijing, China) for 30 min at 37°C. The slides were counterstained with hematoxylin. ImageJ was used to semi-quantify the H-score of immunohistochemistry (IHC) staining.

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